Syntheses via phenolic oxidative coupling using crude peroxidase from Brassica juncea (L) Czern leaves and antioxidant evaluation of dimeric thymol

Authors

  • Yusnidar Yusuf Faculty of Pharmacy and Sciences, Universitas Muhammadiyah Prof. Dr. HAMKA, Islamic Center, Klender, 13460, Jakarta, Indonesia
  • Budi Arman Faculty of Pharmacy and Sciences, Universitas Muhammadiyah Prof. Dr. HAMKA, Islamic Center, Klender, 13460, Jakarta, Indonesia
  • Antonius H. Cahyana Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Kampus UI Depok, 16424, Jawa Barat, Indonesia

Abstract

The ability of a crude Brassica juncea (L) Czern peroxidase as green and edible catalyst for phenolic oxidative dimerization of thymol is presented.  Crude peroxidase was isolated and partially purified using precipitation method with gradient concentration of ammonium sulphate and dry ice-acetone combination as temperature regulator.  The crude peroxidase was then analyzed qualitatively and quantitatively using guaiacol as a model substrate and amino antypirin activity test, respectively, and the positive result was confirmed.  From the phenolic oxidative reaction, dimerization of thymol was carried out under extremely mild reaction condition in aqueous medium.  The product was checked by using Fourier Transform Infrared (FTIR) and Gas Chromatography-Mass Spectrometry (GC-MS).  Furthermore, the biological activity test using DPPH radical scavenging method was confirmed that the dimeric thymol showed 15 times greater than monomeric thymol in term of antioxidant capacity.

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Published

2015-01-08

Issue

Vol. 3 No. 6 (2015)

Section

Bioorganic Chemistry

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