Glucosinolate profiles by HPLC-DAD, phenolic compositions and antioxidant activity of Eruca vesicaria longirostris: Impact of plant part and origin

Authors

  • Saoussen Bouacida 1 University of Carthage, High School of Food Industries of Tunis, Avenue Alain Savary 58, 1003 Tunis, Tunisia 2 Faculty of Sciences of Tunis El Manar, Laboratory of Organic and Structural Chemistry, Campus Universitaire, 2092, Tunis, Tunisia
  • Hayet Ben Haj koubaier 1 University of Carthage, High School of Food Industries of Tunis, Avenue Alain Savary 58, 1003 Tunis, Tunisia 2 Faculty of Sciences of Tunis El Manar, Laboratory of Organic and Structural Chemistry, Campus Universitaire, 2092, Tunis, Tunisia 3High School of Agriculture of Kef, 7119, Le Kef, Tunisia
  • Ahmed Snoussi 1 University of Carthage, High School of Food Industries of Tunis, Avenue Alain Savary 58, 1003 Tunis, Tunisia 2 Faculty of Sciences of Tunis El Manar, Laboratory of Organic and Structural Chemistry, Campus Universitaire, 2092, Tunis, Tunisia
  • Marie Laure Fauconnier Gembloux Agro-Bio Tech, University of Liege, Organic and General Chemistry Department, Passage des Déportés st. 2, 5030, Gembloux, Namur, Belgium
  • Nabiha Bouzouita 1 University of Carthage, High School of Food Industries of Tunis, Avenue Alain Savary 58, 1003 Tunis, Tunisia 2 Faculty of Sciences of Tunis El Manar, Laboratory of Organic and Structural Chemistry, Campus Universitaire, 2092, Tunis, Tunisia

DOI:

https://doi.org/10.13171/mjc55/01606022015/bouzouita

Abstract

The glucosinolate profiles, phenol and flavonoid contents and the antioxidant activity of Eruca vesicaria longirostris were studied for different organs and origins. Eleven desulpho-glucosinolates (DS-GLSs) were isolated and quantified by lipid chromatography- DAD. Similarity between profiles was obtained. Total DS-GLS content, expressed as sinigrin equivalents (SE) revealed a certain variabilily ranging between (76.07-45.61), (27.01-13.53), (4.52 -18.01), (9.39-3.37) and (1.16-13.99) µmol /g DW for seeds, flowers, leaves, roots and stems, respectively. Results showed that seeds are rich in phenolics as they contain highest amounts of phenolics ranging from 27.6±0.5 to 33.47±0.5 mg GAE/g extract as compared to all other parts. Leaves and flowers had a significantly higher total phenolic content than stems and roots in all samples (p < 0.05). According to statistical analysis, the investigated seed extracts with values between (16.20±0.10-18.50±0.10 mg QE/g) exhibited the highest total flavonoids content, followed by leaves (13.00±0.40-15.80±0.30mg QE/g), flowers (10.40±0.40-12.90±0.90 mg QE/g) and stems (7.80±0.20- 9.80±0.70 mg QE/g). Antioxidant activity tested by DPPH, ABTS and FRAP assays, was higher for seeds, leaves and flowers than the other studied organs. These organs were characterized by a significantly high content in glucoerucin, nasturtin and epiprogroitrin, respectively.

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Published

2016-06-02

Issue

Vol. 5 No. 5 (2016)

Section

Food Chemistry

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