Phytochemical study and insecticidal activity of Mentha pulegium L. oils from Morocco against Sitophilus Oryzae

Authors

  • Nadia Zekri Laboratory of Chemistry of Bioactive Molecules and Environment, University of Sciences Moulay Ismail, BP 11201. Zitoune, Meknès, Morocco.
  • Smail Amalich Laboratory of Chemistry of Bioactive Molecules and Environment, University of Sciences Moulay Ismail, BP 11201. Zitoune, Meknès, Morocco.
  • Ahmed Boughdad Département of Plants Protection of Plants and Environement, Ecole Nationale d’Agriculture, BP S/40 ENA, 50000, Meknès, Morocco.
  • Mohamed Alaoui El Belghiti Laboratory of Chemistry - General Physics – University of Sciences- Agdal, 4- Avenue Ibn Battouta. B.P.1014 RP, Rabat, Morocco.
  • Touria Zair Laboratory of Chemistry of Bioactive Molecules and Environment, University of Sciences Moulay Ismail, BP 11201. Zitoune, Meknès, Morocco.

DOI:

https://doi.org/10.13171/mjc.2.4.2013.08.11.23

Abstract

There is a growing interest of industry to replace synthetic chemicals by natural products with bioactive properties from plant origin. The aim of this study was to validate the therapeutic properties of Mentha pulegium L. by conducting a phytochemical study, to determine the chemical composition of its essential oils (EO) and evaluate its insecticidal activity against stored cereals pests. Leaves and flowers of M. pulegium L. were collected from three regions of the Moroccan Middle Atlas. Phytochemical tests on pennyroyal aerial parts revealed the presence of gallic tannins, flavonoids, alkaloids, sterols and triterpenes and saponins. The chemical composition of essential oils was analyzed by gas chromatography coupled with mass spectrometry. The main components were pulegone and piperitenone. The EO from Khénifra is dominated by pulegone (81.46%), and those from Azrou and M'rirt are rich both in pulegone (68.86 and 71.97%) and piperitenone (24.79% and 26.04%) respectively. Pennyroyal oil has showed an important fumigant effect against Sitophilus oryzae (L.) adults. This effect is influenced by the tested doses and exposure periods. The potential of this plant to be used to control stored product insects was discussed.

References

- A. Zargari, Herbal Medicines. 1st Edt, Publication of Tehran University, Tehran. 1990. 14-18.

- A. El Fadl, and N. Chtaina, Etude de base sur la culture de la menthe du Maroc. Programme Régional de lutte intégrée contre les organismes nuisibles (Integrated Pest Management) au Proche Orient. 2010, Report, Office National de sécurité sanitaire des produits alimentaires (ONSSA).

- J.E. Simon, A.F. Chadwick, L.E. Craker, An Indexed Bibliography: 1971–1980: The Scientific Literature on Select Herbs, and Aromatic and Medicinal Plants of the Temperate Zone. Elsevier, 1984, Amsterdam, New York.

- M. Mkaddem, M. Bousaid, and N. Ben Fadhel, Variability of volatiles in 9 Tunisian Mentha pulegium L. (Lamiaceae), Journal of Essential Oil Research. 2007, 19, 211-215.

- A. Bouyanzer, B. Hammouti, and L. Majidi, Pennyroyal oil from Mentha pulegium as corrosion inhibitor for steel in 1 M HCl. Materials Letters. 2006, 60, 2840–2843.

- J.C. Chalchat, M.S. Gorunovic, Z.A. Maksimovic, and S.D. Petrovic, Essential oil of wild growing Mentha pulegium L. from Yugoslavia, J. Essential Oil Res. 2000, 12, 598-600.

- D. Lorenzo, D. Paz, E. Dellacassa, P. Davies, R. Vila, and S. Canigueral, Essential Oils of Mentha pulegium and Mentha rotundifolia from Uruguay. Braz.Arch.Biol.Technol. 2002, 45 (2), 519-524.

- A. Stoyanova, V. Georgie, J. Kula, and T. Majda, Chemical composition of the essential oil of Mentha pulegium from Bulgaria, J.Essential Oil Research. 2005, 17, 475-477.

- A.H. El-Ghorab, The chemical composition of Mentha pulegium L. essential oil from Egypt and its antioxidant activity, J. Ess. Oil Bearing Plant. 2006, 9, 183-195.

- M. Mahboubi, and G. Haghi, Antimicrobial activity and chemical composition of Mentha pulegium L. essential oil Journal of Ethnopharmacology. 2008, 119(2), 325-329.

- N. Benayad, Les huiles essentielles extraites des plantes médicinales marocaines: Moyen efficace de lutte contre les ravageurs des denrées alimentaires stockées. Research Project. University of Sciences, Rabat (Maroc), 2008. 63p.

- H. Hajlaoui, N. Trabelsi, E. Noumi, M. Snoussi, H. Fallah, R. Ksouri, and A. Bakhrouf, Biological activities of the essential oils and methanol extract of tow cultivated mint species (Mentha longifolia and Mentha pulegium) used in the Tunisian folkloric medicine. Springer Science. World. J. Microbiol. Biotechnol. 2009, 25, 2227-2238.

- E. Derwich, Z. Benziane, and A. Boukir, GC/MS Analysis and Antibacterial Activity of the Essential Oil of Mentha Pulegium. J. Agr. Biol. Sc. 2010, 6(3), 191-198.

- A. Boughdad, R. Elkasimi, and M. Kharchafi, Activité insecticide des huiles essentielles de Mentha Sur Callosobrochus maculatus (F) (Coleoptera, Bruchidea). AFPP – Neuvième Conférence Internationale sur les ravageurs en Agriculture. Montpellier 26 and 27 October 2011, 9p.

- J. Bruneton, Pharmacognosie, Phytochimie et plantes médicinales. 4rtEdt. Technique et Documentation. 2009, 1268p.

- B. Marzouk, M. Ben Hadj Fredj, I. Chraief, M. Mastouri, K. Boukef, and Z. Marzouk, Chemical composition and antimicrobial activity of essential oils from Tunisian Mentha pulegium L. Journal of Food, Agriculture & Environment. 2008, 6(1), 78- 82.

- N.H. Jazani, H. Ghasemnejad-Berenji, and S. Sadegpoor, Antibacterial Effects of Iranian Mentha pulegium Essential Oil on Isolates of Klebsiella sp. Pakistan J.Biol.Sc. 2009, 12 (2), 183-185.

-B. Chebli, M. Achouri, L.M. Idrissi Hassani, and M. Hmamouchi, Chemical composition and antifungal activity of essential oils of seven Moroccan Labiatae against Botrytis cinerea, J.Ethnopharmacology. 2003, 89, Issue 1, 165-169.

- S. Hmiri, M. Rahouti, Z. Habib, B. Satrani, M. Ghanmi and M. El Ajjouri, Évaluation du potentiel antifongique des huiles essentielles de Mentha pulegium et d’Eucalyptus Camaldulensis dans la lutte biologique contre les champignons responsables de la détérioration des pommes en conservation. Bulletin de la Société Royale des Sciences de Liège. 2011, 80, 824 – 836.

- L. Alpsoy, H. Sahin, S. Karaman, Anti-oxidative and anti-genotoxic effects of methanolic extract of Mentha pulegium on human lymphocyte culture. Toxicol Ind Health. 2011, 27(7), 647-654.

- G, Franzios, M. Mirotsou, E. Hatziapostolou, J. Kral , G.Z. Scouras, and P. Mavragani Tsipidou, Insecticidal and genotoxic activities of mint essential oils. J. Agric Food Chem., 1997, 45, 2690-2694.

- M. Watt, Natural toxins in traditional medicines some myths removed. Revised 2006; Published first by the Aromatic Thyms (1995), 3(4), 22-30.

- Scientific Committee on Food, Opinion of the Scientific Committee on Food on pulegone and menthofuran. 2002. Report published on http://ec.europa.eu/food/fs/sc/scf/out133_en.pdf

- R. Guba, Toxicity Myths The Actual Risks Of Essential Oil Use. Inter J. Aromatherapy. 2000, 10, Issue 1-2, 37-49.

- E. Kováts, Gas chromatographic characterization of organic substances in the retention

index system. Advances in Chromatography. 1965, 1, 229-247.

- R.P. Adams, Identification of Essential Oils Components by Gas Chromatography Quadrupole Mass Spectroscopy. Allured: Carol Stream, IL. 4rd Edt. 2007. 804p.

- J.B., Harborne, Phytochemical methods. London, Chapman and Hall, Ltd. 3rd Edt. 1998, 320p.

- K. N’Guessan, B. Kadja, G.N. Zirihi, D. Traoré, and L. Aké-Assi, Screening phytochimique de quelques plantes médicinales ivoiriennes utilisées en pays Krobou (Agboville, Côte-d’Ivoire). Sciences et Nature. 2009, 6 (1), 1-15.

- E.L. Kaplan and P. Meier, Nonparametric Estimation from Incomplete Observations. Journal of the American Statistical Association, 1958, 53 (282), 457- 481.

- E.T. Lee, and J.W. Wang, Statistical Methods for Survival Data Analysis, 3rdEdt. Wiley-Interscience, Boulder, CO, USA. 2003, 513p.

- D.J. Finney, Probit analysis. 3rd Edt. Cambridge University Press. London. UK.1971, 333p.

- R. Karousou, D.N. Koureas, and S. Kokkini, Essential oil composition is related to the natural habitats: Coridothymus capitatus and Satureja thymbra in NATURA 2000 sites of Crete. Photochemistry. 2005, 66, 2668-2673.

- M. Kelen, and B. Tepe, Chemical composition, antioxidant and antimicrobial properties of the essential oils of three Salvia species from Turkish flora. Bioresource Technology.2008, 99, 4096- 410.

- M. Ismaili Alaoui, B. Benjilali, and R. Azerad, Application des biotransformations à la valorisation des plantes aromatiques. In : Actes du congrès international sur les plantes aromatiques et leurs huiles essentielles. B. Benjilali, M. Ettaibi, M. Ismaili Alaoui & S.Zrira (eds). 1997, 349-362.

- A. Ait-Ouazzou, S. Lorán, A. Arakrak, A. Laglaoui, C. Rota, A. Herrera, R. Pagán, Evaluation of the chemical composition and antimicrobial activity of Mentha pulegium, Juniperus phoenicea, and Cyperus longus essential oils from Morocco. Food Research International. 2011, 1-7.

- N. Beghidja, N. Bouslimani, F. Benayache, S. Banayache and J C. Chalchat, Composition of the oils from Mentha pulegium Grown in different areas of the east of Algeria, Published in Khimiya Prirodnykh Soedinenii. 2007, 4, 394-395.

- B. Teixeira, A. Marques, C. Ramos, I. Batista, C. Serrano, O. Matos, N.R. Neng, J.M.F. Nogueira, J.A. Saraiva, and M.L. Nunes, European pennyroyal (Mentha pulegium) from Portugal: Chemical composition of essential oil and antioxidant and antimicrobial properties of extracts and essential oil. Industrial Crops and Products. 2012, 36, 81-87.

- G.R. Kabran, N.C. Ambeu, J.A. Mamyrbékova-Békro, and Y.A. Békro, CCM d’extraits sélectifs de 10 plantes utilisées dans le traitement Traditionnel du Cancer du sein en Côte d’Ivoire. Europ. J. Scientific. Research. 2011, 63 (4), 592-603.

- D. D’Abrosca, S. Pacifico, G. Cefarelli, C. Mastellone, and A. Fiorentino, ‘Limoncella’ apple, an Italian apple cultivar: Phenolic and flavonoid contents and antioxidant activity. Food Chemistry. 2007, 104, 1333-1337.

- G. Di Carlo, N. Mascolo, A.A. Izzo, and F. Capasso, 1999. Flavonoids: Old and new aspects of a class of natural therapeutic drugs; Life Sciences, 65 (4), 337-353.

- SA. Abdelgaleil, MI. Mohamed, ME. Badawy, SA. El-Arami, Fumigant and Contact Toxicities of Monoterpenes to Sitophilus oryzae (L.) and Tribolium castaneum (Herbst) and their Inhibitory Effects on Acetylcholinesterase Activity. J Chem.Ecol. 2009, 35, 518–525.

- L.L. Karr, C.D. Drewes, and J.R. Coats, Toxic effects of d-limonene in the earthworm Eiseniafetida (Savigny). Pesticide Biochemistry and Physiology. 1990, 36, 175-186.

- Z. Bouchikhi Tani , M. Bendahou, and M.A. Khelil, Lutte contre la Bruche Acanthoscelides Obtectus et la mite Tineola Bisselliella par les huiles essentielles extraites de deux plantes aromatiques d’Algérie. Lebanese Science Journal. 2010, 11 (1).

- M. El Arch, B. Satrani, A. Farah, L. Bennani, D. Boriky, M. Fechtal, M. Blaghen, and M, Talbi, Composition chimique et activités antimicrobienne et insecticide de l’huile essentielle de Mentha rotundifolia du Maroc. Acta Bot. Gallica. 2003, 150, 267-274.

- S. Hannin, K. Bourarach, M. Ismaili-Alaoui, and B. Benjilali, Activité insecticide de Mentha pulegium vis-à -vis de Rhyzoptera dominica, Sitophilus oryzae et Tribolium castaneum. Actes Editions, Rabat. 1997, 311-315.

- L.S.T. Ngamo, and Th, Hance, Diversité des ravageurs des denrées et méthodes alternatives de lutte en milieu tropical. Tropicultura. 2007, 25(4), 215-220.

- A.F. Ndomo, A.L. Tapondjou, F. Tendonkeng, and F.M. Tchouanguep, Evaluation des propriétés insecticides des feuilles de Callistemon viminalis (Myrtaceae) contre les adultes d’Acanthoscelides obtectus (Say) (Coleoptera; Bruchidae).Tropicultura. 2009, 27(3), 137-14.

- M.D. López, and M.J. Pascual-Villalobos, Mode of inhibition of acetylcholinesterase by monoterpenoids and implications for pest control. Industrial Crops and Products. 2010, 31, 284–288.

- D.N. Price, and M.S. Berry, Comparison of effects octopamine and insecticidal essential oils on activity in the nerve cord, foregut, and dorsal unpaired median neurons of cockroaches. J.Insect Physiology. 2006, 52, 309-319.

Downloads

Published

2013-11-08

Issue

Section

Biological Chemistry