Chemical composition of healthy and infected leaves by Forda riccobonii and Geoica swirskii of Pistacia atlantica Desf. collected from Maaziz region in Morocco


  • Hazim Harouak Soil Microbiology and Environment Unit, Faculty of Sciences, Moulay Ismail University of Meknes, 50 000, Morocco
  • Jamal Ibijbijen Soil Microbiology and Environment Unit, Faculty of Sciences, Moulay Ismail University of Meknes, 50 000, Morocco
  • Touria Zair Laboratory of Chemistry of Bioactive Molecules and Environment, Faculty of Sciences, Moulay Ismail University of Meknes, 50 000, Morocco
  • Laila Nassiri Soil Microbiology and Environment Unit, Faculty of Sciences, Moulay Ismail University of Meknes, 50 000, Morocco



The Atlas Pistachio, Pistacia atlantica Desf., from the family of Anacardiaceae is a widespread species in Morocco and widely present in traditional pharmacopoeia 1. This species is frequently infected by Forda riccobonii and Geoica swirskii  that are foliar galls-inducing aphids.The aim of this study was to compare the chemical composition of essential oils (EO) of the  infected and healthy leaves of Pistacia atlantica. The extraction of essential oils was performed by hydro-distillation using Clevenger, phytochemical screening was realized by revelations tests and chemical composition was identified by gas chromatography coupled with mass spectrometry (GC-MS).Phytochemical analysis showed the presence of some biologically active chemical compounds such as tannins, flavonoids, sterols and triterpenes. The infected leaves were richer in tannins than healthy ones, especially in gallic tannins.Also, the results reaveled that the EO yield of leaves with galls was higher (0.71%) than that of healthy ones (0.11%); also, the first type of leaves was predominated by α-Pinene (19.76%) followed by Terpinen-4-ol, Spathulenol and Sabinene with percentages of 11.94%, 8.90% and 7.63%, respectively. Whereas, healthy leaves were predominated by α-Amorphene (15.05%) followed by Terpinen-4-ol, Spathulenol and α-Muurolene with percentages of 7.17%, 6.46% and 6.20%, respectively.So, we arrived to conclude that infected leaves have a higher level of  monoterpenes hydrocarbon than healthy ones that contains interesting molecules useful in differentes domains, especially in the health field.


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Biological Chemistry