Secondary metabolites and antioxidant activity of seed extracts from Solanum elaeagnifolium Cav.
DOI:
https://doi.org/10.13171/mjc.2.5.2014.05.01.22Abstract
The aim of this study was to screen various solvent extracts of seeds of Solanum elaeagnifolium to display the phytochemical composition, the total phenolic content, the total flavonoid content and the antioxidant capacity in order to find possible sources for future novel antioxidants in food and pharmaceutical formulations. Various extracts of seeds of Solanum elaeagnifolium were obtained by maceration. The total phenolic content of the different extracts was determined by Folin-Ciocalteu method, and the total flavonoid content was quantified using a method based on the formation of a flavonoid–aluminium complex .The antioxidant activity was essayed through some in vitro models such as the antioxidant capacity by phosphomolybdenum method, radical scavenging activity using 2,2-diphenyl-1-picryl hydrazyl (DPPH) assay and reducing power assay. The acetone extract showed the highest total phenolics (580.99 ± 20.56 mg gallic acid equivalents/g of extract), and the highest total flavonoids (207.61 ± 2.62 mg quercetin equivalents/g of extract). This extract showed an antioxidant activity higher than that of α-tocopherol. These results suggest the potential of Solanum elaeagnifolium against free-radical-associated oxidative damage.References
- D.V. Ratnam, D.D. Ankola, V. Bhardwaj, D.K. Sahana, R.M.N.V. Kumar, J. Controlled Release, 2006, 113, 189-207.
- J. Perez-Jimenez, F. Saura-Calixto, J. Agric. Food Chem, 2008, 53, 5036-5040.
- P.K. Agraval, Studies in Organic Chemistry, 1989, 39, 564.
- B.H. Havsteen, Pharmacol. Ther, 2002, 96, 67-202.
- K. Shimoi, S. Masuda, B. Shen, B. Furugori, N. Kinae, Mutat. Res, 1996, 350, 153-161.
- I. Koubaa, M. Damak, Fitoterapia, 2003, 74, 18-22.
- M. Kammoun, I. Koubaa, Y. Ben Ali, R. Jarraya, Y. Gargouri, M. Damak, S. Bezzine, Appl. Biochem Biotechnol, 2010, 162 (3), 662-700.
- N. Damak, N. Allouche, B. Hamdi, M. Litaudon, M. Damak, Nat. Prod. Res, 2011, 26(2), 125-317.
- A. Ben Hsouna, M. Trigui, R. Ben Mansour, R.M. Jarraya, M. Damak, S. Jaoua, Int. J. Food. Microbiology, 2011, 148(1), 66-72.
- S. Miladi, R. Jarraya, M. Damak, J. Applied. Sci, 2008, 8, 4689-4693.
- S. Miladi, N. Abid, C. Debarnôt, M. Damak, B. Canard, M. Aouni, B. Selmi Nat. Prod. Res. 2012, 26(11), 1027-32
- I. Khlif, K. Hamden, M. Damak, N. Allouche, Chem. Nat. Comp, 2012, 40, 799-802.
- N. Gaston, G.P. Guibourt, Histoire naturelle des drogues simples ; ed. by J.-B. Baillière : Pais, 1849.
- D. Cauvet, Des Solanées ; ed. by G. Silbermann: California, 1864, p.75.
- X.M. vander Burgt, J.M. van Medenbach de Rooy, Springer, 1996, 861.
- M. Mellado, J.E. Garcıa, J.R. Arévalo, W. Pittroff, Arid. Environment, 2008, 72, 2034-2039.
- J.W. Boyd, D.S. Murray, R.J. Tyrl, Economic Botany, 1984, 38, 210-217.
- C.A. Chiale, J.L. Cabrera, H.R. Juliani, Phytochem, 1991, 30, 1042-1043.
- D.C. Baker, R.F. Keeler, W. Gaffield, Toxicon, 1989, 27, 1331-1337.
- R.F. Keeler, D.C. Baker, W. Gaffield, Toxicon, 1990, 28, 873-884.
- M.B. Porter, R.J. MacKay, E. Uhl, S.R. Platt, A. deLahunta, J. Am. Vet. Med. Assoc, 2003, 223, 501-504.
- S.0. Trione, M.A. Cony, Interciencia, 1988, 13, 303.
- OEPP/EPPO. Solanum elaeagnifolium, http ://www.eppo.org, (08.04.2010). EPPO Bull, 2007, 37, 236-245.
- A. Bouchelta, A. Boughdad, A. Blenzar, Biotechnol. Agron. Soc. Environ, 2005, 9, 259-269.
- S.Y. Zhang, C.G. Zheng, X.Y. Yan, W.X. Tian, Biochem. Bioph. Res. Co, 2008, 371, 654-658.
- N. Hayder, A. Abdelwahed, S. Kilani, R. Ben Ammar, A. Mahmoud, K. Ghedira, Mutat. Res, 2004, 564(1), 89-95.
- J.B. Harbone, Phytochemical methods: a guide to modern techniques of plant analysis; ed. by Chapman and Hall: London, 1998, p. 302.
- A. Sofowora, Medicinal plants and Traditional Medicine in Africa. Spectrum Books, Ibadan, 1993, pp. 150.
- J.B. Harborne, Phytochemical Methods: A Guide to Modern Technique of Plant Analysis. Chapman and Hall: London, 1973, pp. 49-188.
- G.E. Trease, W.C Evans, Pharmacognosy; ed. by Bailliere Tindall: London, 1989, pp. 176-180.
- A. Djeridane, M. Yousfi, B. Nadjemi, D. Boutassouna, P. Stocker, N. Vidal, Food Chem, 2006, 97, 654-660.
- V.L. Singleton, J.A. Rossi, Am. J. Enol. Vitic, 1965, 16, 144-158.
- P. Prieto, M. Pineda, M. Aguilar, Anal. Biochem, 1999, 269, 337-341.
- W. Brand-Williams, M.E. Cuvelier, C. Berset, Technol, 1995, 28, 25-30.
- Y. Chen, M.F. Wang, R.T. Rosen, C.T. Ho, J. Agr. Food Chem, 1999, 47, 2226.
- G.H. Naik, K.I. Priyadarsini, J.G. Satav, M.M. Banavalicar, P.P. Sohoni, M.K. Biyani, H. Mohan, Phytochemistry, 2003, 63, 97.
- M. Oyaizu, Jpn. J. Nutr, 1986, 44, 307.
- N. Benhammou, F.A. Bekkara, T.K. Panovska, C. R. Chimie 12, 2009, 1259-1266.
- A. Cakir, A. Mavi, A. Yıldırım, M. E. Duru, M. Harmandar, C. Kazaz, J.Ethnopharmacol, 2003, 87,73-83.
- F. Oke, B. Aslim, S. Ozturk, S. Altundag, Food Chem, 2009, 112, 874-879.
- K. Schlesier, M. Harwat, V. Bohm, R. Bitsch, Free Rad. Res, 2002, 36, 177-187.
- D. Yang, Q. Wang, J. Jiang, T. Ying, Asia Pac. J. Clin. Nutr, 2007, 16(1), 158-163.
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